Increased serial levels of platelet‐derived growth factor using hypoxic mesenchymal stem cell‐conditioned medium to promote closure acceler‐ ation in a full‐thickness wound

The healing process of a full‐thickness wound involves complex cascade cellular responses to reverse skin integrity formation. These processes require growth factors, particularly platelet‐derived factor (PDGF). Conversely, hypoxic mesenchymal stem‐cell‐conditioned medium (HMSC‐CM)‐contained factors notably contribute acceleration healing. This study aims investigate the role HMSC‐CM in controlling serial levels PDGF associated with accelerated closure wounds. Twenty male Wistar rats wounds were developed as animal models. animals randomly assigned four groups, comprising two treatment groups (treated using at high dose P1 and low P2), control group (administration base gel), sham (healthy group). examined an enzyme‐linked immunosorbent assay. Using ImageJ software, percentages determined photographically. showed that there was significant increase on days 3 6 after treatment, followed by decrease day 9. In line these findings, percentage also increased significantly rat model, administration may promote increasing wound.